Figure 1.

Screening of the 16 Selected TFs to Evaluate Their Influence on OL Specification

(A) Scheme of the strategy followed for NPC generation, expansion and transduction with lentiviral vectors.

(B) Gene expression changes of early (CSPG4 and OLIG2e), intermediate (ASCL1e and SOX10e), and late (GALC and PLP1) OPC markers measured by qRT-PCR, expressed as fold change relative to control (NPCs transduced with eGFP).

(C) Staining for A2B5 in the different transduced cell progeny on day +7.

(D) Representative images of the A2B5 stainings of vector control and ASCL1 and NKX6-2 transduced cells. Hoechst 33258 (blue) was used as nuclear marker.

(E) Fold change in the expression of the MCS5-SOX10 reporter as a result of the overexpression of the different TFs relative to control (cells transduced with empty vectors) after 7 days in OL differentiation media.

(F) Example of the expression of the MCS5-SOX10 reporter (GFP-FITC) within mCherry⁺ cells in vector control and SOX10-transduced progeny.

Data represented as mean ± SEM of N = 3–4 independent experiments. ^∗p < 0.05. Scale bars: 50 μm.