Figure 7.

All hPSC-Derived OL-Neuron Co-culture Can Address Myelinating Drugs in an HTS Setting

(A) Relative MBP expression to control cultures (0.1% DMSO and no T3) of the different compounds and drugs affecting myelination: T3 (60 ng/mL), DAPT (1 μM), miconazole (1 μM), clobetasol propionate (5 μM) and pranlukast (22.8 μM). Three variables were assessed: total MBP⁺ area, number of MBP⁺ cells and total MBP⁺ intensity. Shown are results for co-cultures in 96-well plates, in 384-well plates, or the combination of the two.

(B) Gaussian dot plots of the average values of all single wells in the six culture conditions (N = 160 for all conditions except for pranlukast, where N = 90) for the three variables based on MBP expression.

(C) Representative images of the co-cultures assessing the effects of the different tested conditions on myelination and of the MBP⁺ mask identified for the analysis.

Hoechst 33258 (blue) was used as nuclear marker. Scale bars: 100 μm. Data are represented as average values per plate ± SEM of each condition of N = 7–8 independent experiments for 96-well plates, N = 6–7 for 384-well plates, and N = 12–15 for both 96- and 384-well plates. ^∗p < 0.05, ^∗∗p < 0.01.