Figure 5.

Interaction of HP1γ with OCT4 and DPPA4 Is Independent of the Presence of HP1α or HP1β and H3K9 Methylation

(A) Immunoprecipitation (IP) of endogenous HP1γ with immunoblot for OCT4 and DPPA4. Asterisk (^∗) marks IgG heavy chain (∼55 kDa).

(B) IP of endogenous DPPA4 with immunoblot for HP1γ.

(C) Confocal immunofluorescence image show regions of colocalization of DPPA4 with HP1γ except in mitotically dividing cells. Scale bar, 10 μm; inset scale bar, 5 μm.

(D) Immunofluorescence of HP1α and HP1β ESC knockout cell lines. Scale bar, 10 μm.

(E) IP of endogenous HP1γ in Crispr wild-type (WT), HP1α KO, and HP1β KO ESC with immunoblot for OCT4 and DPPA4.

(F) Western blot of H3K9me2 and H3K9me3 in FLAG-HP1γ ESC line with V5-tagged H3.3 K9M compared with control V5-tagged H3.3 WT. Cells were treated with doxycycline (Dox) to induce expression of FLAG-HP1γ.

(G) IP of FLAG-HP1γ in (i) H3.3 WT ESC and (ii) H3.3 K9M ESC, with immunoblot for OCT4 and DPPA4.

See also Figure S5.