Figure 1.

Feeder-, Xeno-, and Serum-free Induction of SLCs from hESCs

(A) Crystal violent staining of SLC colonies derived from hESCs (H1) under different conditions. Vc, vitamin C; 2ME, 2-mercaptoethanol. Cell numbers were measured by MTT assay and shown on the right. Relative absorbance of samples at 490–650 nm was calculated. Data are represented as mean of fold changes (compared with the one without Vc and 2ME) ± 1 SEM from three independent experiments.

(B) Flow cytometry analyses of PLZF+ cells on differentiated H1 hESCs under different conditions.

(C) Real-time RT-PCR analyses of germ cell-specific genes at various time points along H1 hESC differentiation. Data are represented as mean ± 1 SEM from three independent experiments.

(D) IF assays of germ cell-specific genes performed at various time points along H1 hESC differentiation. Day 10, D10; Scale bar, 50 μm.

See also Figure S1.