Figure 2.

Human SLCs Undergo Meiosis and Form Putative Haploid Cells

(A) Meiosis DNA spreading assays were performed with antibodies against CENPA and SYCP3 on H1 hESCs and their derivative SLCs at day 11 post-differentiation. Scale bar, 5 μm.

(B) Flow cytometry analyses of percentage of haploid cells formed from H1 ESCs at various time points along differentiation. Mouse testes were used as control.

(C) IF of key proteins that participated in meiosis, including ACR and PRM1, on sorted haploid cells differentiated from H1 ESCs at day 12. Scale bar, 10 μm.

(D) FISH analyses of sex chromosomes and Chr 18 on diploid cells and haploid cells sorted from the same differentiation culture at day 12. Scale bar, 5 μm.

(E) Bisulfate sequencing on imprinting loci of parental H1 ESCs, SLCs, sorted haploid cells at day 12 post-differentiation, and spermatids collected from biopsied human testes. White circles, unmethylated CpG; black circles, methylated CpGs.

See also Figure S2.