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. 2018 Jan 11;10(2):356–365. doi: 10.1016/j.stemcr.2017.12.001

Figure 2.

Figure 2

Insufficient Autophagic Degradation in OPCs Triggered by α-Syn PFF Application

(A) Accumulation of Triton-insoluble endogenous rat α-syn in OPCs is triggered by α-syn PFF (3 μM) application.

(B) Quantitative real-time PCR reveals unchanged Snca expression levels after 72-hr incubation of oligodendrocyte lineage cells with 3 μM α-syn PFFs. Mean ± SEM; n = 6, respectively, independent cultures; paired t test. NS, not statistically significant.

(C) Immunoblot analysis with proteolytic markers discloses marked increases in p62 and LC3-II in OPCs, suggesting the induction of an autophagic pathway due to α-syn PFF application.

(D–G) Quantification of each proteolytic marker expression in oligodendrocyte lineage cell is exhibited. (D and E) Autophagic indicators, p62 and LC3-II/LC3-I, show increasing trends in α-syn PFF-treated OPCs. (F) Cathepsin D protein expression is not significantly affected by α-syn PFF application. (G) The expression of lysine-48-linked ubiquitin chains is not affected by α-syn PFF application. Mean ± SEM; n = 4, respectively, independent cultures; one-way ANOVA, ∗p < 0.05, ∗∗∗p < 0.001.