Figure 1.

Biallelic Integration of FP-SNP Pairs Enable Deterministic Genotype Outcomes

(A) Donor vectors contain a PSM expressing EGFP or dTOMATO, and an NSM expressing tagBFP. PSMs contain puromycin resistance gene (Puro).

(B) Representative example of SNCAe3 polyclone 636. Random integration tagBFP^pos cells are excluded from the correctly edited on-target cells tagBFP^neg.

(C) Theoretical distribution of populations for non-random outcomes.

(D) Representative example of SNCAe3. On-target cells include homozygous populations, EGFP^pos/EGFP^pos or dTOMATO^pos/dTOMATO^pos (type 2), and heterozygous populations of undefined second-allele state EGFP^pos/WT-NHEJ or dTOMATO^pos/WT-NHEJ (type U). WT, wild-type.

(E) Outcomes of the derived population are defined according to the donor vector design.

(F) The tagBFP NSM allows removal of random integration events, assisting in the derivation of defined outcomes.