Figure 3.

Transposase-Mediated Excision of PSMs

(A) FACS analysis for PSM removal. Two transfection steps of excision-only transposase result in removal of the PSMs for SNCAe2 and SNCAe3. Purification of EGFP^neg/dTOMATO^neg cells yields footprint-free edited lines. Diagram of removal population types is shown (right).

(B) Cultures after transposase transfection for SNCAe2 present single and double PSM removal events (in arrowheads) as shown in (A). Scale bar, 200 μm.

(C) Sanger sequencing chromatogram of transposed SNCAe2 p.A30P polyclone 632 and parental control.

(D) Sanger sequencing chromatogram of transposed SNCAe3 p.A53T polyclone 636 and parental control.

(E) Analysis of the respective polyclone composition as in Figure S3.