Figure 4.

Edited SNCA Isogenic Lines Present PD-Associated Phenotypes

(A) NESC differentiation protocol.

(B) Microarray expression level for SNCA, TUBG1, and GAPDH in healthy control NESCs. Data represent three replicates.

(C) Western blot subsequent to denaturing SDS-PAGE for α-synuclein and GAPDH for NESCs.

(D) Wave plot of oxygen consumption rates for the α-synuclein isogenic set. Each wave corresponds to three biological replicates. SD of the sample is included.

(E) Maximal respiration, proton leak, basal respiration, ATP production, and non-mitochondrial respiration for the extracellular energy flux analysis in (D).

(F) Radar plot of fold changes for the parameters in (E). Significance levels correspond to the higher p value assigned to a mutant per category. Significance determined by unpaired Student's t-test.

Significance levels are ^∗p < 0.05, ^∗∗p < 0.01 and ^∗∗∗p < 0.001; n.s., not significant.