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. 2018 Jan 30;2(3):254–269. doi: 10.1002/hep4.1142

Figure 3.

Figure 3

LINC00340/CASC15/TLINC is a novel TGFβ‐induced lncRNA. (A) Analysis of LINC00340 expression in HuCCT1 and Huh28 cells after 16 hours of culture in absence (white bars) or presence (black bars) of 1 ng/mL TGFβ, as evaluated by microarray and Q‐RT‐PCR. (B) Analysis of SMAD7 and LINC00340 expression in Huh28 cells after 72 hours treatment with 10 µM TGFβ inhibitors. (C) Time‐course analysis of LINC00340 expression in HuCCT1 and Huh28 cells (1 ng/mL TGFβ). (D) Analysis of SMAD7 and LINC00340 expression in primary human hepatocytes (n = 3 independent donors) in the presence of 1 ng/mL TGFβ and 10 µM TGFβ inhibitor LY2157299 for 16 hours. (E) Analysis of LINC00340 expression in HCC cell lines (left panel) and LX2 and Panc‐1 (pancreatic cancer) cells (right two panels) (1 ng/mL TGFβ for 16 hours). Statistical analysis was performed by t test (*P < 0.05; **P < 0.01; ***P < 0.001); n ≥ 3 replicates. Data represent mean ± SD. Abbreviations: DMSO, dimethyl sulfoxide; inh., inhibitor; mRNA, messenger RNA; ns, not significant.