Fig 3. ERK differential phosphorylation depends on H₂O₂ in normal murine lung tissue.

Normal male FVB mice (n = 3) were treated with saline solution or 6 mM H₂O₂, both administered intravenously. The detection of pTyr (pYERK) and Thr/Tyr phosphorylated ERK1/2 (2pERK) was analyzed by Western blot. Representative images of the mitochondrial (Panel A) and cytosolic fractions (Panel B) of three independent experiments. Actin and complex III (CIII) antibodies were used as loading control markers to obtain relative phosphorylated ERK levels. Levels of pYERK and 2pERK relative to actin or to CIII expressed in arbitrary units (A.U.). Saline solution-treated mice results were defined as 1 and were used to normalize H₂O₂ samples (inset below images).