Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 30;2(3):329–343. doi: 10.1002/hep4.1145

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors. Hepatology Communications published by Wiley Periodicals, Inc., on behalf of the American Association for the Study of Liver Diseases.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

CDE diet‐induced liver inflammation is reduced in WSX‐1^−/−. Wild‐type and WSX‐1^−/− mice were subjected to the CDE model. (A) Hepatic mRNA expression of macrophage‐related genes was assessed by qRT‐PCR. (B) Immunostaining of F4/80 was performed on WT and WSX‐1^−/− mice, and positive cells were counted after 3 days of the CDE diet. Scale bar, 100 µm. (C) Hepatic mRNA expressions of inflammation‐related genes were quantified by qRT‐PCR; each group n = 4‐7 animals. *P < 0.05, **P < 0.01, ***P < 0.005, WT versus WSX‐1^−/− mice. (D) RAW264.7 cells were cultured in the presence of 50 ng/mL IL‐27, and mRNA expressions of IL‐6, TNF‐α, and IL‐27 subunits were analyzed by qRT‐PCR. *P < 0.05, control versus IL‐27‐treated cells. Data represent mean ± SEM. Abbreviations: CT, control; d, day; qRT‐PCR, quantitative reverse‐transcription polymerase chain reaction.