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. Author manuscript; available in PMC: 2018 Mar 22.
Published in final edited form as: J Am Chem Soc. 2017 Mar 10;139(11):4195–4201. doi: 10.1021/jacs.7b00659

Figure 2.

Figure 2

ClbH-A1 is dispensable whereas ClbH-C–A2–T is required for cyclopropane incorporation. (a) ClbH-A1 loads serine onto carrier protein ClbE, dehydrogenases ClbD and ClbF convert it to α-aminomalonyl-ClbE, and AT ClbG transfers this extender unit downstream. (b) Multidomain signatures of precolibactins 1 and 2. Cellular trans-AT complementation for the ClbI-cis-AT mutant is noted. Domains are color-coded by function, and domains of individual multimodular proteins are grouped together by vertical lines.