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. Author manuscript; available in PMC: 2018 Mar 22.
Published in final edited form as: J Am Chem Soc. 2017 Mar 10;139(11):4195–4201. doi: 10.1021/jacs.7b00659

Figure 3.

Figure 3

Multidomain signatures reveal unexpected biosynthetic events. (A) The thiazoline introduced by ClbJ is oxidized on the assembly line by the downstream Ox domain in ClbK. Module skipping of ClbK-PKS results in diversified products. (B) Accumulation of thiazoline 4 in the Ox mutant supports that ClbK Ox is responsible for oxidizing the thiazoline installed by ClbJ to the thiazole. (C) Module skipping is observed in the multidomain signature of 5. Bithiazole 5 does not require the ClbK-PKS module or the ClbH-A1 domain. Macrocycle 6 requires the ClbK-PKS module for α-aminomalonyl extender unit incorporation.