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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Histol Histopathol. 2014 Oct 10;30(5):559–568. doi: 10.14670/HH-30.559

Figure 4. Splenomegaly with expansion of B-cells and resistance to Fas mediated apoptosis in splenocytes and livers of K1 transgenic mice.

Figure 4

Figure 4

Figure 4

Figure 4

(A) Hematoxylin/eosin (H&E) staining and immunohistochemistry staining of K1and kappa/lambda light chains in spleens from one control (K1-negative littermate) and two K1 transgenic mice; notable are clusters of kappa-positive and scattered single lambda-positive cells; (B) IL-6 (left panel) and CXCR5 (right panel) mRNA levels were analyzed by qRT-PCR in splenic cells isolated from control WT (n=2) and K1-transgenic (n=10) mice; 2 control samples were pooled together; IL-6 average level 4.07 relative to controls (range 1.15 – 7.16); CXCR5 average level 1.24 relative to controls (range 0.62–1.90); (C) Splenocytes from 6 month old K1 transgenic mice and control K1-negative littermates were harvested and incubated with 50 ng/mL of anti-Fas antibody Jo2 for the indicated times. Subsequently, cells were analyzed for apoptosis; (D) K1 transgenic mice and their K1-negative littermates were challenged with a lethal dose of anti-Fas antibody Jo2 and monitored for survival up to 6 hours post challenge.