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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Am Chem Soc. 2018 Jan 22;140(4):1227–1230. doi: 10.1021/jacs.7b12579

Figure 1.

Figure 1

(A) Schematic design of the immunostimulatory SNA. (B) Three distinct linker chemistries were used to make the antigen-DNA conjugates 1–3: NDEC (traceless), SPDP (cleavable), and BMPS (noncleavable), respectively. (C) Cholesterol-modified cyanine-5 (Cy5)-tagged anchor DNA, conjugate and anchor duplex, and SNA were characterized using 1% agarose gel, imaged by Cy5 fluorescence. (D,E) DLS shows an increase in diameter along with a decrease in zeta potential, measured at pH 7, between the bare liposome and the SNAs. Samples for DLS were prepared without the Cy5 modification.