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. Author manuscript; available in PMC: 2018 Apr 12.
Published in final edited form as: Nature. 2017 Oct 12;550(7677):529–533. doi: 10.1038/nature24269

Figure 3. CDX2 overexpression promotes intestinal metaplasia of the transitional basal progenitor cells at the SCJ.

Figure 3

a, CDX2 overexpression in basal cells leads to metaplasia (insert) in the SCJ of Krt5-rtTA; otet-CDX2-T2A-mCherry mice. n=5 per group. b-c, CDX2 overexpression leads to intestinal metaplasia with goblet cells (black arrows) and the loss of p63 protein in some metaplastic cells (white arrows), while the MLE contains p63+ and KRT8+ cells. n=5 per group. d, Metaplastic cells express the intestinal proteins Villin1 and AGR2, and the goblet cell markers MUC2 and TFF3. n=5 per group. e, CDX2 overexpression in KRT7+ cells leads to metaplasia (insert) at the SCJ of Krt7-CreER; R26rtTA; otet-CDX2-T2A-mCherry mice. n=4 per group. f, CDX2 overexpression leads to the loss of p63 protein in the metaplastic epithelium (arrows). Note the presence of MLE with underlying p63+ basal cells and KRT8+ luminal cells. n=4 per group. Scale bar, 50 μm.