Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Cell Rep. 2018 Feb 13;22(7):1647–1656. doi: 10.1016/j.celrep.2018.01.049

Figure 2. Akap200 Is Neuronal and Glial.

Figure 2

(A) Western blot of Akap200-GFP protein trap and control extracts probed with GFP antibodies.

(B) Akap200-GFP is expressed broadly in the adult brain, labeled with GFP (green) and DLG (magenta) antibodies. ctx: cortex region containing glia and neuron cell bodies; al: antennal lobe. Genotype: w,UAS-Akap200.IR/+;Akap200PBss/+. Scale bar: 50 μm.

(B′) Akap200-GFP in the antennal lobe.

(C) Akap200 RNAi in all neurons reduced synaptic neuropil Akap200-GFP.

(C′) Akap200-positive ensheathing glia. Genotype: w,elav(c155)-Gal4,UAS-Akap200.IR/+;Akap200PBss/+.

(D) Akap200 RNAi in all glia reduced Akap200-GFP expression.

(D′) Loss of glial staining in the antennal lobe. Genotype: w,UAS-Akap200.IR/+;Akap200PBss/+;repo-Gal4/+.

(E) Akap200 qPCR for all transcripts in fly heads when Akap200 RNAi is expressed ubiquitously with Act5c-Gal4, normalized to Act5c > +. All bar graphs are mean with SEM.