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. 2018 Mar 1;18:238. doi: 10.1186/s12885-018-4143-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The effects of trastuzumab on HER2 phosphorylation in CHO-K6 cells by immunofluorescence. CHO-K6 cells were treated with trastuzumab at concentrations ranging from 0.1–10 μg/ml for 1 h. The phosphorylation of HER2 at Y1005 (a), Y1112 (b), Y1127 (c), Y1139 (d), Y1196 (e), and Y1248 (f) were then examined by immunofluorescence as described in Methods. The localization of trastuzumab was revealed by TRITC-conjugated donkey anti-human IgG. The localization of HER2 was revealed by rabbit anti-phosphoHER2 antibody followed by FITC-conjugated donkey anti-rabbit IgG. The cell nuclei were counter stained with DAPI. Yellow indicated the co-localization of trastuzumab and phosphoHER2. The cells treated with normal human IgG (10 μg/ml) were used as negative controls. Size bar: 10 μm