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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Adv Healthc Mater. 2017 Sep 25;6(23):10.1002/adhm.201700641. doi: 10.1002/adhm.201700641

Figure 5.

Figure 5

Western blot of intracellular signaling molecules expressed by hMSCs cultured on Col-GAG and MC-GAG in the absence and presence of PD98059. Western blot of A) phosphorylated Smad1/5 (P-Smad1/5) and total Smad (Smad5), phosphorylated ERK1/2 (P-ERK1/2) and total ERK1/2 (ERK1/2), phosphorylated JNK1/2 (P-JNK1/2) and total JNK1/2 (JNK1/2), Runx2 and B) phosphorylated p38 (p-p38) and total p38, phosphorylated Akt (p-Akt) and total Akt, and actin in hMSCs cultured on Col-GAG and MC-GAG at days 0, 4, 14, and week 4 of culture with and without 50 μm PD98059.