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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2017 Oct 26;37(12):2260–2270. doi: 10.1161/ATVBAHA.117.310290

Figure 1.

Figure 1

SEC Isolation of nHDL. nHDL was collected from the media after apo AI incubation of BHK cells over expressing ABCA1 with no FC-loading (A, 0 CDX) and with FC-loading with 2.5 mM (B) and 5.0 mM CDX (C) and analyzed by SEC. Grey-filled plots are the A280 SEC of the media; red bars denote the fractions from media that were collected and pooled as large (L), medium (M) and small (S) nHDL. Analytical A280 SEC of the pooled fractions L, M and S are shown in the chromatograms labeled a, b and c respectively. Non-denaturing PAGE (D) and SDS-PAGE (E) of the L, M and S pooled fractions from each of the three cell cholesterol loading conditions as labeled. Molecular weight standards are in the left lanes, and apo AI and BSA are as labelled.