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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2017 Oct 26;37(12):2260–2270. doi: 10.1161/ATVBAHA.117.310290

Figure 2.

Figure 2

Transfer of nHDL-[3H]FC to Serum Lipoproteins. nHDL-[3H]FC was prepared from BHK-ABCA1 cells labeled with [3H]FC as in Figure 1. nHDL-[3H]FC were tested under several conditions and analyzed by SEC, which was monitored by β-counting of the collected fractions. A) nHDL-[3H]FC; B) nHDL-[3H]FC incubated with HDL at 4 °C and immediately analyzed by SEC (0 min). C) nHDL-[3H]FC incubated with serum at 4 °C or 37oC for various times as labeled and analyzed by SEC. D) Kinetics of [3H]FC accumulation in LDL (fractions encompassed by grey box in Panel C). E) The kinetics of nHDL-[3H]FC transfer to isolated LDL determined by heparin-Mn+2 precipitation of LDL.