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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: Cell. 2018 Jan 25;172(4):811–824.e14. doi: 10.1016/j.cell.2017.12.038

Figure 6. Generation and characterization of ADAR1 KO hESCs.

Figure 6

(A) Immunofluorescence staining of WT and ADAR1 KO hESCs. SSEA4 (green), Oct-3/4 (red), and DAPI (blue). Scale bar, 100um.

(B) hESC growth rate/day measured by the CellTiter-Glo assay.

(C) qRT-PCR of IFNβ and ISGs. Data shown as mean ± SEM (n = 3~6 experimental replicates).

(D) Western blot of mock and IFNβ treated (24 hours) hESCs. p-PKR, PKR phosphorylation.

(A–D) Par., parental hESC clone. WT, CRISPR/Cas9 derived WT hESC cell clone. ADAR1 KO #1 and #2 are two independent CRISPR/Cas9 derived ADAR1 KO hESC clones (see Table S2).