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. Author manuscript; available in PMC: 2019 Jul 5.
Published in final edited form as: Anal Chim Acta. 2018 Feb 5;1012:1–9. doi: 10.1016/j.aca.2018.01.037

Fig. 2.

Fig. 2

Summary of the results from the mouse brain proteome digest using SCX-RPLC-CZE-MS/MS. Three salt steps were employed for step-wise elution of peptides from the SCX to the RPLC. (A) The accumulated numbers of protein group and unique peptide IDs vs. the number of fractions. A 71-cm separation capillary was used for CZE-MS/MS. (B) Comparison of the number of protein group and unique peptide IDs from the twenty LC fractions corresponding to the second salt step analyzed by the CZE-MS/MS with a 71-cm separation capillary (short) or a 92-cm separation capillary (long). (C) An electropherogram of one SCX-RPLC fraction analyzed by CZE-MS/MS with the 92-cm separation capillary. The migration time and the full width at half maximum (FWHM) of three peptides were shown in the figure.