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. 2018 Feb 28;13(2):e0193277. doi: 10.1371/journal.pone.0193277

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© 2018 Hwang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A) Heat-killed E. coli OP50 compared to live E. coli OP50. B) Heat-killed E1: O104 compared to live E1: O104. C) Heat-killed E3: O111 compared to live E3: O111. D) Heat-killed E12: O121 compared to live E12: O121. E) Heat-killed E15: O145 compared to live E15: O145. F) Heat-killed E18: O145 compared to live E18: O145. G) Heat-killed E22: O157 compared to live E22: O157. The survival curves of nematodes feeding on both heat-killed STEC strains and the E. coli OP50 strain were examined. The live strains were compared to the heat-killed strains. All heat-treated bacterial strains used in this assay increased the lifespan of C. elegans when compared to the control strains. This suggests that the toxic factors of STEC strains are heat-labile.