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. 2018 Feb 28;13(2):e0193277. doi: 10.1371/journal.pone.0193277

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© 2018 Hwang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Conventional PCR: Based on the results of the life span assay, 6 STEC strains (L1—E1; L2—E3; L3—E12; L4—E15; L5—E18; L6—E22; L7 –OP50) out of the 24 strains were selected for further testing for the presence of toxin genes (stx1, stx2 and groEL) using 3 primer pairs (STX1-F/R-O157; STX2-F/R-O157; GroEL-F/R-O157) targeting the genes specific for toxin production(stx1, stx2 and groEL). Colonisation assay: The STEC strains colonised in the intestinal tract of L2 worms (log CFU/worm). p<0.001 was obtained for comparison with the E. coli OP50 control group by the t-test. The total numbers of worms (C. elegans) tested in each group are indicated by n.