. 2018 Feb 28;13(2):e0193277. doi: 10.1371/journal.pone.0193277

Table 2. Oligonucleotide primer sequences used in this study with respect to product size.

Targeting genes	Primers	Primer Sequence (5’→3’)	Product Size(bp)	Annealing Temperature (°C)	Source
stx1	STX1-F-O157	`GAAAGCGATGCAGCTATTA`	789	60	Chou et al., 2013
stx1	STX1-R-O157	`GGATAATTTGTTTGCAGTTG`	789	60
stx2	STX2-F-O157	`TATTATTTAAATGGGTACTGTGC`	1073	60
stx2	STX2-R-O157	`ATGTGTCATCCTCATTATACTTG`	1073	60
groEL	GroEL-F-O157	`CCGTAACGTAGTTCTGGATA`	1493	60
groEL	GroEL-R-O157	`CTAAGTCAGCTGCATCGTT`	1493	60