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. 2018 Feb 28;13(2):e0193371. doi: 10.1371/journal.pone.0193371

Fig 3. Validation of differentially expressed miRNAs by qRT-PCR.

Fig 3

A. Result of RNAseq. B. Result of qRT-PCR. oar-miR-10a, oar-miR-148a, oar-miR-200b oar-miR-133 and oar-miR-194 were known sheep miRNAs, cgr-miR-142-5p, chi-miR-215-5p, bta-miR-1388-5p, age-miR-15b, bta-miR-2478, and hsa-miR-141-3p were conserved miRNAs, novel_miR_17, novel_miR_147 and novel_miR_217 were novel miRNA candidates. The relative quantification of expression was calculated using the 2-△△CT method after the threshold cycle (Ct) and was normalized with the Ct of U6. The relative expression levels were presented as the 2-△△CT means ± SE. The error bars indicate the standard error of the 2-△△CT mean values. * represents p <0.05, ** represents p <0.01.