Skip to main content
. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Appl Toxicol. 2017 Apr 19;37(9):1108–1116. doi: 10.1002/jat.3465

Figure 1.

Figure 1

Environmental PFOS exposure promotes dolphin T cell division. (A) Representative dot plots depicting the gating strategy used to identify sized (SSC vs. FSC-A), single (FSC-H vs. FSC-A), live (LiveDeadneg), CD4+ and CD8+ T cells in dolphin peripheral blood leukocytes. (B) Illustration of CFSE staining profile of CD4+ and CD8+ T cells from peripheral blood leukocytes at day 0 and following 4 days incubation with exogenous PFOS. Numbers represent the percentage of cells in each gate. (C) CD4+ and CD8+ T cell proliferation following 4 days culture in the absence of exogenous PFOS. Extent of CD4+ and CD8+ T cell division is shown in groups of dolphins segregated by tertiles of plasma PFOS (upper graphs) and PFHpA (lower graphs). CFSE, carboxyfluorescein succinimidyl ester; FSC-A, forward scatter-area; FSC-H, forward scatter-height; PFHpA, perfluoroheptanoate; PFOS, perfluorooctane sulfonate; SSC, side scatter. [Colour figure can be viewed at wileyonlinelibrary.com]