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. Author manuscript; available in PMC: 2019 Mar 2.
Published in final edited form as: Virus Res. 2018 Jan 31;247:26–33. doi: 10.1016/j.virusres.2018.01.010

Figure 3.

Figure 3

The effects of siRNA treatment targeting PDI1, PDIA3, PDIA4 or Grp78 on the replication influenza A and B viruses. A) Relative mRNA levels of target or β-actin gene following siRNA treatment was calculated using the relative standard curve quantitation method by plotting ct values with standard curves generated using serial dilutions of each mRNA in MDCK cells. The numbers indicate the means and the standard deviations of the means of the relative target mRNA levels. B) Effects of siRNA treatment targeting irrelevant, PDI1, PDIA3, PDIA4 or Grp78 on the replication influenza A and B viruses. One-day old MDCK cells in 12-well plates were transfected with mock-medium (Mock), irrelevant siRNA (irre, 100 nM), or target siRNA at 100 or 10 nM. At 48 hr post-transfection. Cell lysates were collected and total RNA was extracted for real time qRT-PCR for each target gene or β-actin and then the RT-PCR products were visualized on the agarose gel.