Table 1.

Sequences of siRNA and real time qRT-PCR primers and probe for PDI1, PDIA3, PDIA4 and Grp78 used in this study.

siRNA	Target	Sequences (duplex of synthetic RNA)
	PDI1	rGrArU rCrUrC rArGrA rArCrC rUrUrC rUrGrC rCrUrU rCrArG rCrUrU*
		rArGrC rUrUrC rUrCrU rGrCrC rArGrC rUrGrU rGrUrA rCrUrC rCrCrU
		rCrCrA rUrCrC rUrUrG rCrUrC rArGrC rUrGrG rUrArU rUrUrG rGrArG
	PDIA3	rArUrC rCrUrC rArArU rGrGrC rArUrC rUrUrC rUrUrU rArUrU rGrGrA *
		rUrGrA rGrArG rGrArA rCrUrG rArGrG rCrUrG rGrUrC rCrUrG rCrCrU
		rUrGrU rGrArA rGrGrA rCrGrA rArArC rArArG rGrUrG rArUrA rCrCrC
	PDIA4	rCrUrC rCrUrC rUrUrC rCrUrC rGrUrC rGrUrC rGrUrC rCrUrC rCrUrC *
		rCrArA rUrArA rArGrA rArGrA rUrGrC rCrArU rUrGrA rGrGrArU
		rGrGrA rGrGrA rCrGrA rCrGrA rCrGrA rGrGrA rArGrA rGrGrArG
	Grp78	rUrArG rUrGrA rGrArA rCrCrA rUrGrG rCrArG rArArA rUrUrU rCrUrU *
		rArCrU rCrArA rUrUrU rCrArA rUrUrC rUrUrG rCrUrU rGrArU rGrCrU
		rCrArA rGrUrG rUrUrC rCrArG rArUrC rUrCrG rGrUrU rUrCrC rGrUrC
Real time qRT-PCR	Target	Sequences (5′ to 3′ DNA)
	PDI1	FWD-TCA AGG GCA AGA TCC TGT TTA T
		REV-CGG GCA CTC CTC TTT CTT TAG
		Probe-/56-FAM/TT CAT CGA C/Zen/A GCG ACC ACA CTG AC/3IABkFQ/
	PDIA3	FWD-GCC AGC AAC TTG AGG GAT AA
		REV-GGA AGG ACG AAA CAA GGT GAT A
		Probe-/56-FAM/CC GGT TTG C/Zen/A CAC ACC AAT GTT GA/3IABkFQ
	PDIA4	FWD-CTA ACA GCC TGA GAG AGG ATT AC
		REV-CGT AGC TCT TGG GCT CAT ATT
		Probe-/56-FAM/TG CAG CCT G/Zen/A GAA ATT CCA GTC CA/3IABkFQ/
	Grp78	FWD-GTT CTT GTT GGT GGC TCT ACT
		REV-ACA GCC TCA TCT GGG TTT ATG
		Probe-/56-FAM/TT TAA TGG C/Zen/A AGG AGC CAT CCC GT/3IABkFQ
	β-actin	FWD-GGCATCCACGAAACTACCTT
		REV-AGCACTGTGTTGGCGTACAG
		Probe-/HEX-ATCATGAAGTGTGACGTGGACATCCG-TAMRA

^*The listed siRNAs were confirmed to reduce both virus replication and target mRNA.