Skip to main content
. Author manuscript; available in PMC: 2019 Mar 2.
Published in final edited form as: Virus Res. 2018 Jan 31;247:26–33. doi: 10.1016/j.virusres.2018.01.010

Table 5.

Effects of the siRNA treatment for PDI1, PDIA3, PDIA4 or Grp78 on the replication of A/WS/33 in MDCK cells determined by real time qRT-PCR at 2 and 12 hr after virus inoculation.

siRNA Relative viral RNA levels compared to mock transfection*
A/WS/33
2 hr 12 hr
Mock (water) 100 100
PDI1 (P4HB, PDIA1) 92 (3.3) 9 (1.4)#
PDIA3 (Erp57) 96 (1.9) 12 (3.1) #
PDIA4 (Erp72) 103 (2.7) 7 (2.9)#
Grp78 (BiP) 94 (3.8) 18 (4.3)#
Irrelevant siRNA 102 (2.7) 93 (2.7)
*

The relative RNA levels of the virus M gene following the siRNA treatment were determined as percentage relative to the mock transfection following normalization with β actin.

#

Statistically significant differences from the mock (p < 0.05). The numbers in parentheses are standard deviations of the means.