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. Author manuscript; available in PMC: 2019 Mar 2.
Published in final edited form as: J Mol Biol. 2018 Jan 10;430(5):695–709. doi: 10.1016/j.jmb.2018.01.002

Fig. 6.

Fig. 6

2D 13C–13C PDSD spectra of the TMD with long mixing times of 0.5 and 1.0 s, measured at 253–263 K. Only inter-residue cross peaks are assigned. (a) AGILV (L493F)-labeled TMD in the POPC/cholesterol membrane. (b) 1:1 mixture of AGILV (L493F) and ILSILV-labeled TMD in the POPE membrane. All inter-residue cross peaks are intramolecular ones. (c) Undiluted and 1:2 diluted (green) ILSILV-labeled TMD in the POPC/cholesterol membrane. The same inter-residue cross peaks were observed, indicating that all cross peaks are intramolecular. (d) Mixed GV and IS-labeled TMD in the POPE membrane, showing I501–S505 cross peaks. (e) Intramolecular Cα–Cα distances of the TMD generated using the measured (φ, ψ) torsion angles.

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