Table 1.
13C, 15N-labeled (red underlined) and fluorinated (blue bolded) residues in the PIV5F TMD (485–510) peptides used in this study
Samples | Labeled residues | POPC/ Chol |
POPE | |
---|---|---|---|---|
1 | IAGLSV: I488, A490, G497, L498, S505, V506 |
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x | x |
2 | 1:1 mixed GV and IS G494, V506 and I501, S505 |
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x | x |
3 | ILSILV: I491, L493, S495, I499, L500, V508 |
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x | x |
4 | AGILV (L493F): A492, 4- 19F-L493F, G494, I502, L503, V507 |
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x | x |
5 | 1:1 mixed ILSILV and AGILV (L493F) |
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- | x |
6 | Diluted ILSILV | ILSILV : Unlabeled peptide = 1: 2 | x | - |
7 | L504F |
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x | - |
8 | L500F |
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x | - |
Sample 1 (IAGLSV) was used in a previously reported study [32], while samples 2–8 are produced specifically for this study.