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. 2018 Mar 1;18:29. doi: 10.1186/s12935-018-0527-x

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Cell viability analysis of H295R cell line treated with everolimus (Eve) alone or with mitotane (Mit). MTS assay in monolayer cells after everolimus treatment for 3 days (a) and (b) a combination of Eve and 10 μM Mit for 72 h; (c) MTS assay in spheroids after treatment with Eve or a combination of Eve and Mit; d phase-contrast photomicrography of spheroids after 72 h of treatment; e Confocal microscopy images of spheroids after 72 h of treatment (100 μm). The results are expressed as the mean ± SEM; ANOVA **p < 0.01; ***p < 0.001; ****p < 0.0001; n = 3