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Cell viability analysis of ACC-T36 cell cultures treated with nilotinib (Nil) alone or with mitotane (Mit). MTS assay after Nil treatment for 3 days or a combination of Nil and 10 μM Mit in monolayer cells (a) and (b) in spheroid preparations; c phase contrast photomicrography of spheroids treated for 72 h and control cells (100 μm) stained with Oil Red O. The results are expressed as the mean ± SEM; ANOVA **p < 0.01; ***p < 0.001; ****p < 0.0001; n = 3
