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. 2018 Mar 1;13:10. doi: 10.1186/s13024-018-0242-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Dynein/dynactin complex remains intact in Dctn1^LoxP/LoxP; Cre/Esr1 neurons. a-b Sucrose density gradient centrifugation shows p150^Glued and p135+ predominantly migrate at fraction 4 and 5 in a 5–20% sucrose gradient similar to the other dynactin subunits p62, p50, and ARP1, as well as the dynactin-interacting dynein subunit DIC in the control Dctn1^LoxP/LoxP (a) and p150^Glued cKO Dctn1^LoxP/LoxP; Cre/Esr1 cortical neuron cultures (b) at 7 DIV