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. 2018 Mar 1;9:52. doi: 10.1186/s13287-018-0798-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Osteogenic differentiation of hMSCs cultured on 62–68 kPa ECM. a Cells plated on indicated substrates for 1 week and then subjected to morphological analysis, Alizarin Red and alkaline phosphatase (ALP) staining. Scale bar = 20 μm. b Expression of osteoblast markers COL1AI, RUNX2, and BGLAP measured by qRT-PCR after 1, 2, and 3 weeks of culture. *P < 0.05. n = 3. TCP tissue culture plates, COLIAI alpha-1 chains of type I collagen, RUNX2 Runt related transcription factor 2