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Validation of the identified miRNA existence and their expression by northern blotting. a U6 snRNA served as the loading control. Csa-U6–1 and csa-U6–2 using total RNA extracted from embryos and adult total RNA, respectively. b Northern blot analysis of csa-miR-4018a, csa-miR-4018b, csa-miR-4040, csa-miR-4086, and csa-miR-4000f was performed in denaturing gels using LNA probes. The size of mature miRNAs was approximately 21 nucleotides
