Figure 9.

Regulation of laccase by miR408. (a) mRNA levels of the putative target of miR408 was analyzed by real-time PCR in mock-inoculated plants, Acidovorax avenae-infected plants; (b) using Puccinia kuehnii-infected plants that showed different degrees of symptoms in comparison to the expression in asymptomatic plants; and (c) using sugarcane hybrid SP70-1143 and two wild species inoculated with a beneficial diazotrophic bacteria, Gluconacetobacter diazotrophicus. Dotted line represents the expression of the control samples. The error bars represent the standard deviation between three technical replicates. * represents significant differences in expression between the control and inoculated samples for each experiment (p-value < 0.05); (d) Alignment of miR408 and its putative target. The red arrow shows the cleavage site confirmed by 5′ RACE (Rapid Amplification of cDNA Ends), and the number is the amount of clones that present this cleavage site (in parentheses) in a total of clones that were sequenced.