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(a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.
