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. 2016 Oct 25;2(4):12. doi: 10.3390/ncrna2040012

Figure 2.

Figure 2

Cytoplasmic/perinuclear colocalization of Dicer and TWEAK proteins in human cells. Flag-TWEAK and human Dicer proteins were expressed in cultured HeLa cells prior to confocal immunofluorescence microscopy. TWEAK was labeled with a rabbit polyclonal anti-FLAG and a secondary anti-rabbit-IgG coupled to AlexaFluor 546 fluorophore (in red), whereas Dicer was labeled using a monoclonal anti-Dicer antibody and a secondary murine anti-IgG coupled to AlexaFluor 488 fluorophore (in green). Cell nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI). Proteins were visualized using a confocal microscope (Quorum spinning Disc Wave Fx, Quorum Technologies, Guelph, ON, Canada) and a 63× objective. DIC, differential interference contrast.