Figure 3.

Modulatory effects of the conditioned medium from MSCs on cytokines sciatic nerve levels of neuropathic mice. The naïve group consists of mice that did not receive any experimental manipulation, while the sham group represents mice in which the sciatic nerve was exposed but left intact. Seven days after PSL, mice were treated by endovenous route with bone marrow-derived mesenchymal cells (MSCs; 1 × 10⁶/100 μl), conditioned medium from MSCs (CM; 100 μl), or vehicle (control medium; 100 μl). Panels shows the sciatic nerve levels of (a) interleukin-1β (IL-1β), (b) tumor necrosis factor-α (TNF-α), (c) interleukin-6 (IL-6), and (d) interleukin-10 (IL-10), determined in ipsilateral sciatic nerve samples by ELISA at day 21 after the PSL surgery. The results are expressed as picograms of cytokine per milligram of protein. Data are expressed as means ± SEM; n = 6 mice per group. ^∗Significantly different from the vehicle group (p < 0.05). ANOVA followed by Tukey's multiple comparison test.