Figure 4.
Biochemical assessment for antiosteoporosis effect of rutin in vitro. SAOS-2 cells were treated with rutin (1 μM) and E2 (0.1 μM) for 48 h and compared to the control untreated cells. Biochemical assessment of osteoporosis was evaluated by measuring alkaline phosphatase (a), osteocalcin (b), vitamin D3 (c), and acid phosphatase (d). Data are presented as mean ± SD; n = 3. ∗Significantly different from the control untreated cells; p < 0.05.