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. 2018 Jan 31;2018:9517842. doi: 10.1155/2018/9517842

Figure 3.

Figure 3

Th1 preponderance in the coculture system derived from mice of abortion-prone matings. Flow cytometry measurements of Gal-9 protein on PBMCs. We show intuitive images with the green line representing the addition of PE-Gal-9-Ab and the black line representing the addition of IgG-control (a). The mean fluorescence intensity showed a downregulated Gal-9 expression on PBMCs from abortion-prone matings (b). PBMCs were cocultured with trophoblasts. IL-2 (c), IFN-γ (d), and IL-4 (e) were evaluated in coculture by ELISA at d 1, d 3, and d 6. There was higher IL-2 and IFN-γ production and lower IL-4 production from abortion-prone matings than from controls at d 1, d 3, and d 6 (n = 5). ∗∗p < 0.01; ∗p < 0.05.