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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Am J Sports Med. 2017 May 25;45(11):2637–2646. doi: 10.1177/0363546517706138

Figure 5.

Figure 5

Collagen type 2 gene transcript and matrix deposition in human amnion-derived mesenchymal stromal cells and epithelial cells (hAMSCs and hAECs) and human adipose-derived stromal cells (hADSCs). (A) Immunohistochemical staining (brown = positive, blue = nuclei) for collagen type 2 in hAMSC, mixed (hAEC + hAMSC co-culture), and hADSC cell pellets cultured in chondrogenic differentiation medium. (B) Gene transcript expression levels of collagen type 2 in mixed (hAEC + hAMSC co-culture) and hAMSC groups relative to hADSCs (dotted line) cultured in basal medium. (C) Collagen type 2 expression of amnion-derived mesenchymal stromal cells cultured in chondrogenic differentiation medium compared with respective expression in hADSCs cultured at the same time points. Lines indicate statistical difference (P < .05) between connected groups. *Statistical differences compared with the hADSC group at the same time point (P < .05).