TABLE 1.

Osteogenic and Chondrogenic Gene Transcript Summary Information

Gene Name	Species	Gene Symbol	Amplicon Size	Polymerase Chain Reaction Protocol
Aggrecan	Human	ACAN	62 bp	Activation: 95°C for 15 minutes
Sex Determining Region y-box 9	Human	SOX9	111 bp	Cycling (45 cycles):
Type II Collagen	Human	COL2A1	94 bp	Denaturation: 94°C for 10 seconds
Runt Related Transcription Factor 2	Human	RUNX-2	101 bp	Anneal: 52.4°C for 20 seconds
Osteocalcin	Human	OCN/BGLAP	90 bp	Extension: 72°C for 20 seconds
Glyceraldehyde 3-Phosphate Dehydrogenase	Human	GAPDH	95 bp	Melt Analysis: 72°C–99°C at 1°C per step
Detected Transcript (NCBI Reference Sequence)

ACAN: NM_001135, NM_013227, XM_001131727, XM_001131734, XM_006720419

SOX-9: NM_000346

COL2A1: NM_001844, NM_033150, XM_006719242

RUNX-2: NM_001015051, NM_001024630, NM_004348, NM_001278478, XM_006715231, XM_006715233, XM_006715234

OCN/BGLAP: NM_199173

GAPDH: NM_001256799, NM_002046, NM_001289745, NM_001289746