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. 2018 Feb 14;2018:5180165. doi: 10.1155/2018/5180165

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Copyright © 2018 Jianan Geng et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The activity of MMP-2. (a) The activity of MMP-2 was detected by zymography; MMP-2 will hydrolyze the gelatin at corresponding position and reveal transparent visible band under blue background; the brightness of band represents the activity of the enzyme. 62 KD brand is the active form of MMP-2; 72 KD brand is the form of pro-MMP-2. (b) The relative value of MMP-2 and pro-MMP-2 activity. Enzyme volume = strip area × (band gray − background gray). The results were standardized to MMP-2 enzyme volume and normalized to 1.0 in control group. Con: nondiabetic control group; DN: streptozotocin-induced diabetic group; HACE-L: low-dose-HACE-treated group; HACE-H: high-dose-HACE-treated group; Bena: benazepril. ^∗∗P < 0.01 versus Con; ^▲▲P < 0.01 versus DN.