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. 2018 Feb 26;8:3584. doi: 10.1038/s41598-018-22085-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flagellar nanotube formation from FliC-aGFP_ENH_V2 subunits in vivo and in vitro. (a) Bright-field TEM image of Salmonella cells that possess mutant flagellar filaments. Samples were stained with 2% phosphotungstate to enhance image contrast. (b) Filaments polymerized from purified subunits as visualized by dark-field optical microscopy. Polymerization experiments were carried out in PBS buffer (pH 7.4) at 2 mg/ml protein concentration and 4 M AS was added to 0.6 M final concentration to initiate filament formation.