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. 2018 Feb 26;8:3580. doi: 10.1038/s41598-018-22044-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of the protective efficacities in a lethal doses S. aureus peritonitis model. Female BALB/c mice (n = 10) were peritoneally challenged with (A) S. aureus Newman (8 × 10⁸ CFUs), (B) S. aureus Wood46 (1 × 10⁸ CFUs) and (C) S. aureus HLJ 23-1 (5 × 10⁸ CFUs) after immunization. (D) Immunotherapy, MntC-vaccinated mice 1 h after S. aureus Newman (8 × 10⁸ CFUs) infection. Survival rates were daily monitored and recorder for 14 days, and compared to animals treated with PBS. Significant differences were indicated by (*P < 0.05), (**P < 0.01) and (***P < 0.001).